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Abstract1 i. i- h' a, N9 r: l6 e
Aligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar
, P5 I9 U' m8 A# N9 E- P5 JNMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation
7 B7 U. U! B7 h3 Quniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles9 m t, e8 r3 A7 q
are advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity! N# j: d0 g$ z3 K
to living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe
$ D1 C+ s; A% Xin a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully
; j6 ?/ ?7 k6 Q: s1 P! C9 f& Brecorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity.
7 Y, @! ~; B; CThe total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation., U/ p7 J: p' w1 M* c5 J
This model predicts the signal-to-noise ratio and the progression of the nutation curves.
% n+ T9 i9 K& _1 p+ P3 Q4 a 2005 Elsevier Inc. All rights reserved.
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3 u+ N9 Y K; S/ J) T/ n, e[ 本帖最後由 mt7344 於 2007-6-7 10:38 PM 編輯 ] |
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