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Abstract
) a! \8 {) Q" V H4 J2 ] Z& c2 cAligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar
8 ]0 s: {: e& W5 |# ?/ \NMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation
- ~ o# \+ J/ Nuniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles1 I% I/ p3 z, l* S5 w+ t7 v+ c, ]- \
are advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity; X5 ^ K/ F2 p8 c9 x" D
to living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe
2 I$ A( C+ ^) t" w1 qin a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully* k* e- G0 F! d( A2 b) O" U
recorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity.
4 U1 q2 J+ f' P. S( xThe total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation.( t; C; M. P0 G+ k) e8 j3 f
This model predicts the signal-to-noise ratio and the progression of the nutation curves.
^0 ^6 S: Y; u: }2 `: ] 2005 Elsevier Inc. All rights reserved.
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* h& U& c2 ~" \7 R網路上抓的 paper, 希望對大家有幫助!!+ B5 Y2 o- x: @5 ^
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[ 本帖最後由 mt7344 於 2007-6-7 10:38 PM 編輯 ] |
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